Effect of type 2 diabetes mellitus on lithium-pilocarpine-induced status epilepticus in adult rats.
Abstract number :
1.397
Submission category :
Camelice Poster
Year :
2021
Submission ID :
1886517
Source :
www.aesnet.org
Presentation date :
12/4/2021 12:00:00 PM
Published date :
Nov 22, 2021, 06:56 AM
Authors :
Karen Ramos Riera, PhD - Brain Research Institute; María Leonor Lopez Meraz, Dr - Brain Research Institute; Luis Beltran Parrazal, Dr - Brain Research Institute; Pablo Martinez Gopar, PhD - National Institute of Neurology and Neurosurgery; Francisca Perez Severiano, Dr - National Institute of Neurology and Neurosurgery; Carol Merida Portilla, PhD - Student, Brain Research Institute
Rationale: Status epilepticus (SE) is an emergency neurological condition, and there is evidence that diabetic hyperglycemia can present it. However, the mechanisms involved in hyperglycemia and the susceptibility to ES and the effects in neuronal death are not yet clear. The Objective is study the effect of type 2 diabetes mellitus (DM2) on status epilepticus severity (SE), neurodegeneration, and brain oxidative stress (OS) in adult rats.
Methods: DM2 was induced in male Wistar rats with streptozotocin (STZ, 100 mg/kg, s.c, n=18) (1) on the postnatal day (P)3; control rats (CTRL, n=19) were administered with vehicle (0.1 M citrate buffer citrate, pH 4.5, s.c.). In P90, SE was induced in both groups with the lithium-pilocarpine model (3 mEq/kg, i.p. LiCl; 30 mg/kg, s.c, pilocarpine hydrochloride). Fluoro-Jade B staining was used to analyze neurodegeneration 24 h after SE. Lipid peroxidation and the formation of reactive oxygen species (ROS) were evaluated in the hippocampus, the thalamus, the amygdala, and the piriform cortex 6 and 24 h after SE (2).
Results: STZ rats showed increased latency to the first generalized seizure (35.6± 2.3 min) and SE (39.4±1.9 min) compared to CTRL rats (26.6±1.5 min y 29±1.5 min, respectively). STZ rats also exhibited a higher number of generalized seizures (5.1± 0.3) of longer duration (0.9 ±0.08 min) compared to CTRL rats (3.4±0.1 y 0.6±0.06 min, respectively). STZ rats displayed a higher number of F-JB cells in the hippocampus (416.6±34.9), the amygdala (515.4± 41.8), the thalamus (483.0± 21.6), and the piriform cortex (1485±84.2) in comparison with CTRL rats (248.2± 30.9, 355.0± 24.3, 285.0± 14.8 y 813.0± 33.6, respectively). Brain OS levels were similar in STZ and CTRL groups.
Conclusions: DM2 increases the severity of SE and neurodegeneration in adult rats.
Funding: Please list any funding that was received in support of this abstract.: This study was supported by CONACyT [PhD scholarship awarded to KPRR (893823)].
Camelice Poster