Abstracts

S.E. can produce brain damage. Propofol is an anaesthetic with anticonvulsant effect , but its use is limited by complications such as Rhabdomyolisis There are no experimental studies that can demonstrate neuroprotective activity of Propofol in S.E, neither R. attributed to this drug .

1 [ndash] To evaluate if Propofol in an animal model of Pilocarpine [ndash] induced S.E can prevent neuronal damage in hippocampal structures.
2 [ndash] To evaluate if Propofol in S.E. in this experimental model can cause R., Adult male Wistar rats, ( 200 to 300 g) were used. Biosecurity rules defined by the Institute of Basic Sciences and Experimental Medicine of our Hospital were followed.
: N= 9 rats: 4 cm3 saline solution intraperitoneal ( i.p)
: N=10: 1mg /kg N-Hyoscine butylbromide+ 350 mg/kg of i.p Pilocarpine.
: N= 1mg /kg N-Hyoscine butylbromide+ 350 mg/kg of i.p Pilocarpine. After 30 minutes of S.E., 20 mg/kg Propofol.
Behaviour and Scalp EEG monitoring were evaluated. Brains were removed after 2 hours,and stained with hematoxylin-eosin (H.E ).
The hippocampus was assessed.
4 histologic groups with signs of neuronal damage(dark and shrunken) were identified .




The muscle quadriceps femoralis was removed and stained with H.E. The presence of macrophage in and around the fibres was evaluated .
The pathologist performed analyses blinded to the treatment received by the rats., Brain damage in every area was analyzed with a non-parametric test (Wilcoxon sum rank).A p[lt] 0.05 was considered a significant difference.
Between Group1 and Group 2, greater difference was found in temporal neocortex and subiculum in Group 2. Significant damage decrease was observed in Group 3, with less damage in the CA3-4 regions (p=0.0019).
[underline]BEHAVIOUR[/underline]
All the rats had S.E.
[underline]EEG[/underline]:
Group1: normal EEG.
Group 2: continuous epileptiform activity .
Group 3: continuous epileptiform activity during S.E. Propofol produced suppression of the epileptiform activity.
[underline]R.:[/underline] 2 rats in each group(Group 2 and Group 3) had signs of R.There were no statiscally significant differences between treatment groups., Neuronal damage was less severe in the rats treated with Propofol , with decrease neuronal damage regarding the CA region, specifically in the CA3-4 regions, which suggests its neuroprotective effect. R. was not considered a statiscally significant finding.,