EFFECTS OF UPP ON PSD95/NR2B EXPRESSION IN RATS WITH PILOCARPINE-INDUCED EPILEPSY
Abstract number :
2.326
Submission category :
11. Human Genetics
Year :
2009
Submission ID :
10035
Source :
www.aesnet.org
Presentation date :
12/4/2009 12:00:00 AM
Published date :
Aug 26, 2009, 08:12 AM
Authors :
Yangmei Chen, J. Zhang and Z. Zhang
Rationale: To investigate the expression and the potential role of PSD95/NR2B on epileptogenesis in hippocampus of rats with epilepsy and to explore the effect of Ubiquitin-proteasome pathway (UPP) on the expression of PSD95/NR2B of epileptic rats. Methods: The expression of PSD95/NR2B in hippocampus was examined by the immunofluorescent analysis. Using Histopathological stain to detect morphological changes in hippocampus. Results: The expression of PSD95/NR2B was gradually decreased in hippocampus of pilocarpine-induced epileptic rats. The down-regulation of PSD95/NR2B expression in hippocampus had been inhibited in the MG-132 pretreated rats(P<0.05, VS. PILO group) and the degeneration and necrosis of neurons were more severe compared with epileptic rats. Conclusions: Ubiquitin-proteasome pathway plays an important role in the down-regulation of PSD95/NR2B expression in hippocampus of epileptic rats. The down-regulation of PSD95/NR2B expression is considered to be a protective mechanism in epileptogenesis.
Genetics