Expression of the Wnt/β-catenin pathway in hippocampus of rats with kindling model-induced seizures subjected to caloric restriction
Abstract number :
1.416
Submission category :
1. Basic Mechanisms / 1D. Mechanisms of Therapeutic Interventions
Year :
2022
Submission ID :
2232917
Source :
www.aesnet.org
Presentation date :
12/3/2022 12:00:00 PM
Published date :
Nov 22, 2022, 05:28 AM
Authors :
Eric Uribe, BM – Instituto Nacional de Neurología y Neurocirugía MVS; Ernesto Ochoa, BM – Neurofisiología – Instituto Nacional de Neurología y Neurocirugía MVS; Moisés Rubio-Osornio, PhD – Neuroquímica – Instituto Nacional de Neurología y Neurocirugía MVS; Vanessa Mena, BM – Neurofisiología – Instituto Nacional de Neurología y Neurocirugía MVS; Noel Gallardo, BM – Neurofisiología – Instituto Nacional de Neurología y Neurocirugía MVS; Leonardo Hernández, BS – Neurofisiología – Instituto Nacional de Neurología y Neurocirugía MVS; Carmen Rubio, PhD – Neurofisiología – Instituto Nacional de Neurología y Neurocirugía MVS
Rationale: Epilepsy is one of the most prevalent neurological diseases in the world and it is known that approximately 30% of the cases are refractory to pharmacological treatments. For this reason, several alternative treatments have been investigated to promote better control of such cases, such as caloric restriction (CR). CR triggers a complex chain of metabolic and energetic changes that stabilize neuronal dynamics. Different cellular and molecular pathways have been described as mechanisms that explain these changes; a little studied pathway that is altered in epilepsy and that may have therapeutic implications is the Wnt/β-catenin pathway. It is necessary to know the behavior of this pathway in structures vulnerable to epileptic phenomena such as the hippocampus and whether therapies such as caloric restriction have an impact on it.
Methods: Male Wistar rats (n=24) were implanted with electrodes by stereotaxic surgery following the Atlas of Paxinos and Watson. The rats were divided into 4 groups: control (n=6), kindling group (n=6), kindling group with caloric restriction (n=6) and caloric restriction group without kindling (n=6). Subsequently, the rats were sacrificed by intracardiac perfusion and decapitation for the identification of Wnt/ β-catenin pathway proteins. ImageJ software was used for densitometric analysis of protein bands and ANOVA and Tukey's test for statistical analysis.
Results: The results show an overexpression of proteins of the Wnt/ β-catenin pathway in the groups induced by the kindling model (β-catenin, p=0.004; cyclin D, p=< 0.001; GSK3β, p=< 0.001; Wnt, p=< 0.001). In the kindling group with caloric restriction the protein concentrations are similar to that of the control groups, while the Sham caloric restriction group has no significant differences with the Kindling/caloric restriction group.
Basic Mechanisms