Hippocampal RNA Expression Varies According to Seizure Outcome and Seizure Frequency Following Anterior Temporal Lobectomy with Amgydalohippocampectomy
Abstract number :
3.048
Submission category :
1. Translational Research: 1C. Human Studies
Year :
2017
Submission ID :
349877
Source :
www.aesnet.org
Presentation date :
12/4/2017 12:57:36 PM
Published date :
Nov 20, 2017, 11:02 AM
Authors :
Robert Bina, University of Arizona; Ryan Sprissler, University of Arizona, Genetics Core, Division of Biotechnology; Christina Walter, University of Arizona, Div of Neurosurgery; David Labiner, Department of Neurology, Banner University Medical Center; Mi
Rationale: Previous studies have demonstrated meaningful differences in the expression of temporal cortical genes in patients with temporal lobe epilepsy (TLE) that are seizure-free after anterior temporal lobectomy with amygalohippocampectomy (ATL/AH) compared to those with continued seizures after surgery. The current study was conducted to test the hypothesis that RNA expression varies between patients who are seizure-free and those who continue to have seizures after ATL/AH. Additionally, the hypothesis that RNA expression differs between patients with differing preoperative seizure frequency was tested. Methods: Hippocampal tissue from 14 selected patients all of whom underwent ATL/AH for medically intractable TLE was collected. RNA isolates were analyzed and patient seizure outcome and frequency were compared. RNA expression from patients who were seizure-free were compared to those who were not. Also, RNA expression from patients with high baseline seizure frequency were compared to patients with low seizure frequency. Results: 50% of patients were seizure free post-operatively at last follow-up (12-53 months, average 31). 113 and 186 hippocampal genes were upregulated or downregulated respectively when seizure outcomes following ATL/AH were compared – including Transferrin (1.63 log2fold change; p-11), Radixin (1.09 log2fold change; p0.00005), ), and CCL2 (-1.4 log2fold change; P Conclusions: Presented here is evidence that the expression of hippocampal RNA differs in patients when their post-operative seizure outcome is compared – there are hundreds of genes with up or down regulation correlating to post-operative seizure free outcome. Also presented are possible hippocampal genetic markers of seizure frequency. Genes with changes in RNA expression in both of the investigated groups include known and unknown genes. Though the contribution of each gene to the overall outcome is yet to be determined, these genes and data present targets for future research and patient identification for successful ablative procedures. Funding: None
Translational Research