Leukocyte RNA Expression Predicts Human Temporal Lobe Epilepsy Seizure Frequency
Abstract number :
1.016
Submission category :
1. Basic Mechanisms / 1B. Epileptogenesis of genetic epilepsies
Year :
2021
Submission ID :
1826712
Source :
www.aesnet.org
Presentation date :
12/4/2021 12:00:00 PM
Published date :
Nov 22, 2021, 06:56 AM
Authors :
Neil Joshi, MD - University of arizona;
Rationale: This study was performed to test the hypothesis that systemic leukocyte RNA expression has prognostic value differentiating low from high seizure frequency refractory temporal lobe epilepsy (TLE).
Methods: A consecutive series of sixteen patients with refractory temporal lobe epilepsy was studied. Based on a median baseline seizure frequency of 2.0 seizures per month, low versus high seizure frequency was defined as less than or equal to 2 seizures/month and greater than 2 seizures/month, respectively. Systemic leukocyte RNA expression was analyzed for prognostic value for TLE seizure frequency. All differentially expressed genes were analyzed with Ingenuity® Pathway Analysis (IPA®) to identify biological pathways with predictive value for low versus high TLE seizure frequency and for functional annotations wherein clustering of low seizure frequency associated RNAs were significantly over-expressed.
Results: There were ten males and six females with a mean age of 39.4 years (range: 16 to 62 years, standard error of mean: 3.6 years). There were five patients in the high and eleven patients in the low seizure frequency cohorts, respectively. Based on a threshold of 2-fold change (p < 0.001, FC > 2.0, FDR < 0.05), 13 differentially expressed leukocyte genes were identified which were all upregulated in the low and downregulated in the high seizure frequency group, including NCF2 (Neutrophil Cytosolic Factor 2, p67phox), HMOX1 (Heme Oxygenase 1), RHOB (Ras Homolog Family Member B; Rho-Related GTP-Binding Protein RhoB), FCGR2A (FcγRIIA, Fc Fragment of IgG Receptor IIa, CD32), PRKCD (PKCδ, Protein Kinase C Delta), RAC2 (Family Small GTPase 2), TLR1 (Toll Like Receptor 1), CHP1 (calcineurin homologous protein 1), TNFRSF1A (TNFR1, Tumor Necrosis Factor Receptor Superfamily Member 1A; p55), IFNGR1 (Interferon Gamma Receptor 1, IFN-γ R1), LYN (LYN Proto- Oncogene, Src Family Tyrosine Kinase), MYD88 (MYD88 Innate Immune Signal Transduction Adaptor), and CASP1 (Caspase 1, Apoptosis-Related Cysteine Protease, Interleukin-1β Convertase).
Basic Mechanisms