Abstracts

Rationale: Methylation is a key epigenetic modification of DNA and it is argued, "that DNA methylation may play an important role in seizure susceptibility and maintenance of the disorder". DNA methylation status of Grin2B, BDNF, KCC2, NKCC1 associated with refractory temporal lobe epilepsy was investigated in our study. Methods: There were 68 participants in the study. Thirty eight patients with temporal lobe epilepsy (TLE) who was diagnosed by video EEG monitoring and 30 healthy control subjects included in the study. Twenty three of them were men and remaining fifteen of them were women. Twenty seven of them had unilateral temporal focus (nine with right; eighteen with left ) while eleven patients had bilateral TLE. Genomic DNA samples, obtained from peripheral blood, were applied bisulfite PCR and cleanup. Related gen regions were reproduced with PCR primers and sequencing with pyrosequencing method. Results: There were not found methylation on the genes BDNF and KCC2; however were found NKCC1 in 8 patients and in 5 healthy subjects; and Grin2b methylation was also found in one patient. A significant different was not detected about methylation of mentioned genes between patients and control group. There is no association between right or left and unilateral or bilateral onset of temporal lobe. Methylation on the NKCC1 in patients with temporal lobe epilepsy was not dependent febrile convulsion history and mesial temporal atrophy on cranial magnetic resonance imaging. Conclusions: Limited findings were found about DNA methylation in temporal lobe epilepsy. Therefore further studies, that contain large number of patients, will necessary . Funding: No. I will support by ucb pharma for congress