Neuronal Degeneration in the Piriform Cortex and Claustrum in Developing Rats Induced by Status Epilepticus.
Abstract number :
2.023
Submission category :
Year :
2001
Submission ID :
2328
Source :
www.aesnet.org
Presentation date :
12/1/2001 12:00:00 AM
Published date :
Dec 1, 2001, 06:00 AM
Authors :
R. Druga, Institute of Physiology, Academy of Sciences, Prague, Czech Republic; H. Kubov[aacute], Institute of Physiology, Academy of Sciences, Prague, Czech Republic; P. Mare[shacek], Institute of Physiology, Academy of Sciences, Prague, Czech Republic
RATIONALE: To describe topography of neuronal degeneration in the piriform cortex and the claustrum in young rats after status epilepticus (SE).
METHODS: Wistar rats (P12, 15, 18, 21 and 25) pretreated with lithium chloride (3mmol/kg) were injected by pilocarpine (40 mg/kg). Brain sections were stained with Fluoro-JadeB at various survival intervals (from 2 h to 1 week) and examined under fluorescent microscope.
RESULTS: In the P12 rats a small number of degenerating neurons was evident in caudal two thirds of the piriform cortex (mainly in the IIIrd layer), in the ventral endopiriform area and in the ventral claustrum up to 12 h after SE. The number of labeled neurons increased at 24 h following SE. The labelled neuronal profiles were scarce in the P15 pups up to 24 h.
A high number of labelled neurons was found in the piriform cortex, in the ventral endopiriform area, in the ventral and dorsal claustrum of P18, 21, and 25 rats up to 1 week after SE. No labelled neurons were found in age-matched controls.
CONCLUSIONS: Neuronal degeneration induced by SE in piriform cortex and adjacent structures is dependent on age. It is present even in P12 rats but it is more marked since P18 (e.g.in the dorsal claustrum positive neurons were evident only since this age).
Support: Supported by the Grant Agency of the Czech Republic, Grant No 304/99/0193.