Neuroprotective effects of Maqui Berry on blood-brain barrier damage in a model of Pentylenetetrazole-induced recurrent seizures in old rats.
Abstract number :
1.393
Submission category :
Camelice Poster
Year :
2021
Submission ID :
1886419
Source :
www.aesnet.org
Presentation date :
12/4/2021 12:00:00 PM
Published date :
Nov 22, 2021, 06:56 AM
Authors :
Omar García, PhD - IMSS; Angélica Vega, PhD - Physiology - UNAM; Cristina García, PhD - Food Science and Technology - CEBAS-CSIC; Diego Moreno, PhD - Food Science and Technology - CEBAS-CSIC; Sandra Orozco, PhD - Unit of Medical Research in Neurological Diseases - IMSS
Rationale: The aging process progressively deteriorates health and quality of life, increasing susceptibility to seizures. Additionally, blood-brain barrier (BBB) dysfunction has been reported as a cause of the development of seizure activity. The Maqui Berry (MB), Aristotelia chilensis, contains flavonoids with strong antioxidant activity; the inhibition of oxidative stress has anticonvulsant effects.
Methods: Male Sprague dawley rats, 12 months of age, were administered 100 mg/kg of MB, orally, for 20 days before and during the evaluation of seizure susceptibility; a subthreshold dose of Pentylenetetrazol (PTZ) 55 mg/kg (i.p) was applied, volume of 1 ml/kg of body weight three times in 2-day intervals between each evaluation. The BBB proteins,iNOS, Cox-2 and Zo1, were evaluated by Western blot and immunofluorescence in cerebral capillaries, as well as the expression of gliosis and neuronal survival in the ventral hippocampus using GFAP and Neun, respectively.
Results: The MB group showed an increase in the latencies of generalized seizures, a higher expression of Zo1 proteins (p < 0.001), a lower expression of iNOS, Cox-2 in cerebral capillaries compared to the PTZ control group, with a reduction in gliosis and greater neuronal survival in CA3 and GD.
Camelice Poster