Abstracts

Rationale: Oligodendrocyte provides the myelin sheath to insulate the axon in central nervous system. Oligodendrogenesis and myelination are critical steps in neural development, and plays the fundamental role in neuronal plasticity and the proper function of neural networks. On the other hand, neuronal activity also modulates oligodendrogenesis and myelination. It remains unclear whether and how neuron-oligodendrocyte interaction sculptures epileptogenesis in focal cortical dysplasia (FCD) type II, the most common underlying pathology in pediatric drug-resistant epilepsies.

Methods: We generated a clinically-relevant DEPDC5-related FCDII rat mode using CRISPR in utero electroporation. Oligodendrocyte-lineage cells were labeled by Olig2, PDGFα-R, NG2, and CC1.

Results: As compared to the control, the density of Olig2+ cells increased by 45.74 ± 2.20% (n=3) in the dysplastic cortex. The density of cells positive for PDGFα-R or NG2 staining was also increased in the dysplastic cortex. These changes were only evident after animals developed their seizures.

Conclusions: These findings indicate that oligodendrogenesis and myelination are increased in FCDII, providing new mechanistic insights into the pathogenesis of FCDII.

Funding: Please list any funding that was received in support of this abstract.: Y.W. received funding from NIH (K08NS099379, R01NS113824, U54NS117170). S.H. received funding from the Wisdom Accumulation and Talent Cultivation Project of the Third Xiangya Hospital of Central South University and China Postdoctoral Science Foundation (2020M672519, PC2021098).