PREVENTIVE EFFECT OF KD ON THE NEURONAL DAMAGE PRODUCED BY PILOCARPINE-INDUCED STATUS EPILEPTICUS IN RATS
Abstract number :
1.102
Submission category :
Year :
2003
Submission ID :
2232
Source :
www.aesnet.org
Presentation date :
12/6/2003 12:00:00 AM
Published date :
Dec 1, 2003, 06:00 AM
Authors :
Jae-Moon Kim, Hee-Dong Park, Ki-Young Chung, Dong-Wook Kim Neurology, Chungnam National University Hospital, Daejeon, Republic of Korea; Neurology, Samsung Medical Center, Seoul, Republic of Korea; Pediatrics, Ilsan Paik Hospital, Ilsan, Korea
Ketogenic diet (KD) shows an outstanding antiepileptic effect in some epileptic patients and favorable effect on the cognition of these patients. This study was performed to evaluate the neuroprotective and anti-epiletogenic effect of KD.
In the preliminary experiment, to select proper animal model of SE, evaluation of EEG and behavioral characteristics of pilocarpine-induced SE model was done in Sprague-Dawley rats. After the determination of EEG stages in this model, serum nitric oxide levels were measured at four different stages of SE in the KD rats and regular diet (RD) rats. After three weeks of KD/RD diet, SE was induced and treated at each stages of SE with 10-20 mg/kg i.p. of diazepam. Previous diet was maintained for two weeks until the evaluation of pathological changes with H-E and C-V staining. To define whether the KD and RD causes different mossy fiber sprouting, another 80 rats were fed with KD and RD for three weeks and induced SE. After the treatment of SE, half of previous KD rats maintained with KD and remaining 20 rats were fed with RD. Also previous RD rats were fed with RD and KD in the same pattern. Timm[apos]s staining was done 4 weeks after the SE in half of the rats. Remaining rats were monitored every two hours a day for four weeks to count the number of spontaneous recurrent seizures.
KD did not affect the SE induced by pilocarpine in terms of the SE induction time and SE severity. Serum NO was consistently higher in KD rats than RD rats in all SE stages. Neuronal damages in the CA1, CA3, and dentate gyrus were less prominent in the KD rats in every SE stages. Rats with KD before their SE showed less significant Timm[apos]s (+) bands, whereas KD after the SE did not show any differences. Spontaneous recurrent seizures were reduced in the KD rats after the SE.
KD significantly increases NO during the pilocarpine-induced SE and consistently protect neuronal damage caused by seizures in the hippocampus. These neuroprotection causes less mossy fiber sprouting and late seizures.
[Supported by: Korea Research Foundation Grant. (KRF-2001-001-F00114)]