Abstracts

Rationale: Kainic acid (KA) treatment causes neuronal injury primarily in the amigdala and hippocampus as well as many regions of the rat brain and activates epileptic seizures. This model is used to research human temporal lobe epilepsy (TLE) in chronic term. We aimed to investigate clinic and electrophysiologic features of seizures in the KA-treated rats to form TLE models in acute term. Methods: Sixteen young adult Wistar albino rats (220-270 g) were used in this study. Rats were divided into two groups as acute kainic acid and sham. Teflon coated steel wire electrodes were stereotactically inserted into the rat hippocampus bilaterally. A week after electrode implantation, Intraperitoneal (i.p.) injections of KA ( 5-15 mg/kg) were given to rats in order to follow with video-EEG monitorization.Results: The first clinic seizure latency was measured as 85 m (29-132 m) while first seizure duration was 95 s (14-428 s). 7 of 16 rats underwent convulsive status epilepticus (SE) clinically whereas one of them had nonconvulsive SE. One of them died during SE. 27 absence, 35 focal motor, 49 myoclonic seizures were observed in 24 hours. Isolated or serial epileptiform discharges have been detected in the interictal period. No ictal seizure and interictal epileptiform discharges were observed in the sham group. Conclusions: The first study to show the video-EEG synchronization of acute term KA-treated TLE models, a new semiological classification is made by classifying clinic and electrophysiologic seizures.