Role of Alpha2-Adrenergic Neurotransmission in The Anticonvulsant Effects of Cannabinoids on The Pentylenetetrazole-induced Clonic Seizure in Mice
Abstract number :
1.247
Submission category :
7. Antiepileptic Drugs
Year :
2011
Submission ID :
14661
Source :
www.aesnet.org
Presentation date :
12/2/2011 12:00:00 AM
Published date :
Oct 4, 2011, 07:57 AM
Authors :
M. Ghasemi, H. Shafaroodi, K. Amini, A. R. Dehpour
Rationale: Cannabinoid system plays a pivotal role in the seizure threshold modulation which is mainly mediated through activation of the cannabinoid CB1 receptor. There is also several evidence of interaction between cannabinoid system and other neurotransmission systems such as adrenergic system. However, the role of adrenergic system in the anticonvulsant effects of cannabinoids has not been completely demonstrated. In this study using ?2-adrenoceptor agonist (clonidine) and antagonist (yohimbine), the involvement of ?2-adrenergic neurotransmission was evaluated in the effects of the cannabinoid receptor agonist ACEA (arachidonyl-2-chloroethylamide) on the pentylenetetrazole (PTZ)-induced clonic seizure.Methods: PTZ-induced clonic seizure threshold was determined by inserting a 30 gauge butterfly needle into the tail vein of male Swiss mice (23-29 g) and the infusion of PTZ (0.5%) at a constant rate of 1 ml/min to unrestrained freely moving animals. Infusion was halted when forelimb clonus followed by full clonus of the body was observed. Minimal dose of PTZ (mg/kg of mice weight) needed to induce clonic seizure was considered as an index of seizure threshold. The one-way or two-way analysis of variance (ANOVA) followed by Newman-Keuls post hoc test was used to analyze the data. P < 0.05 was considered the significance level between the groups.Results: Acute ACEA administration (0.1-4 mg/kg, i.p.) significantly (
Antiepileptic Drugs