Seizures following intracerebral hemorrhage in the rat collagenase model
Abstract number :
674
Submission category :
2. Translational Research / 2D. Models
Year :
2020
Submission ID :
2423015
Source :
www.aesnet.org
Presentation date :
12/7/2020 9:07:12 AM
Published date :
Nov 21, 2020, 02:24 AM
Authors :
Charlotte Germonpré, Ghent University; Silke Proesmans - Ghent University; Charlotte Bouckaert - Ghent University; Ella Vanhaverbeke - Ghent University; Kristl Vonck - Ghent University; Paul Boon - Ghent University; Robrecht Raedt - Ghent University; Veer
Rationale:
Intracerebral hemorrhage (ICH) is a known risk factor for the development of acute symptomatic seizures and late unprovoked seizures ( > 7 days post-stroke). An animal model to study these post-ICH seizures is currently lacking. The aim of this study was to investigate the occurrence of seizures in the rat collagenase model using long-term video-EEG monitoring (VEM).
Method:
Male Sprague-Dawley rats (n=16) were implanted with 4 scalp electrodes (4 AP ±2 ML and -4.5 AP ±3 ML) and a craniotomy (0.5 AP 3.5 ML) for later injection of collagenase was made and filled with bone wax. After one week of baseline VEM, 0.6 U of collagenase was injected (6 DV) in left striatum via the earlier performed craniotomy. Immediately following ICH-induction, the rats were reconnected to the VEM for 180 days to assess the occurrence of clinical and electrographic seizures. Severity of clinical seizures was scored following the Racine scale.
Results:
Acute symptomatic seizures occurred in 6/16 animals (38 %), with the first seizure starting between 6 and 80 hours after ICH induction. Behavioral changes could be observed in all acute symptomatic seizures. Mean seizure duration was 122±19 s. One animal had a status epilepticus (SE) after 56 hours which lasted for more than 24 hours. After 180 days of VEM, late unprovoked seizures were present in 5/16 animals (31%), of which two animals also had acute symptomatic seizures (including the SE animal). The late unprovoked seizures started between 13 and 172 days post-ICH and had a mean seizure duration of 60±17 s. These late unprovoked seizures were all clinical and more severe than the acute symptomatic seizures (Racine scale 4.7±0.1 vs 3.9±0.2, Mann-Whitney U test p< 0.0001).
Conclusion:
Both acute symptomatic and late unprovoked seizures were frequently seen in the rat collagenase model. Therefore this model seems suitable to study post-ICH seizures and post-ICH epilepsy.
Funding:
:C.G. is supported by a grant of Research Foundation Flanders (FWO, grant 1181719N) and is laureate of the Helaers Research Prize for Stroke.
Translational Research