Abstracts

Our service is based on human epileptogenic tissue study, and since the begining we try to solve the problem of hippocampal survival for [italic]in vitro[/italic] studies. In this paper we show our techinique padronization of surgical ressection and its impact on hippocampal survival. We studied 55 patients of our service with mesial temporal sclerosis and divided them in two groups: with (16) or without (39) hippocampal ressection padronization; and compared the hippocampal survival for [italic]in vitro[/italic] studies. We divided the hippocampal ressection in five steps: first, the temporal horn of lateral ventricle openning and hippocampal exposure; second, anterior ressection of intralimbic girus and amigdala, preserving the head of hippocampus; third, posterior delimitation of hippocampus ressection at the level of body-tail transition; fourth, aspiration of parahippocampal girus; and fifth, medial ressection close to fimbria fornix where vascularization was cuted and hippocampus was delivered to neurophisiology team. The hippocampus ressected before padronization had 38,5% survival against 75% in those after padronization, showing an outstanding improvement in its survival. In our experience the padronization of ressection of human hippocampus was really important to garantee the hippocampal survival for [italic]in vitro[/italic] studies. The time reduction of ischemia during the surgical ressection was remarkable to improve the hippocampus survival index and it was directly related with the padronization of ressection techinique.[figure1][figure2] (Supported by UNIPETE (Epilepsy Research and Treatment Unit)
UNIFESP (Federal University of Sao Paulo))