Abstracts

Rationale: To determine (and compare) the effetcts of valproic acid (VPA), carbamazepin (CBZ), oxcarbazepine (OXC), levatiresetam (LEV), topiramat (TPM), lamotrijin (LTG) on uterine implantation of non-epileptic rats Methods: Total of 147 rats, which had pregnancy 3 to 6 times before, were divided into three main groups (49 each). The subgroups were furher divided into 7 groups. For 30 days, while following the estrus cycles, six different antiepileptic drug were applied by gavage to different groups: VPA (300 mg/kg/day), CBZ (154 mg/kg/day); OXC (30mg/kg/day); LEV (50 mg/kg/day); TPM (100 mg/kg/day); LTG (10 mg/kg/day) and drinking water (for control). The drug applications were made in two doses. Following the drug applications, females were put together with male and vaginals smears were tested. The day vaginal plaque or sperm in vaginal area was observed, was accepted as the 0th day of implantation. On 5th day for the 1st main group, on the 7th day for the 2nd main group and on the 9th day of the 3rd main group the uterus including the embrios were taken out and the embryos were counted. The implanted embryos were immunohistochemically processed and laminin, kollajen IV and vimentin were investigated Results: Although statistically insignificant, in groups received VPA, CBZ and OXC, the number of embryos were reduced (p>0.05). In the same experimental groups emryonic trofoblast cells and lumen epithelial cells of the uteruses exhibited weaker stainging intensities (p<0.05). For analysis Standard HSCORE =? Pi (i 1) was used Conclusions: Antiepileptic drugs especially VPA, CBZ ve OXC may reduce the number of embryos and campared to LEV, TPM and LTG, have stronger negative effects on rat uterus implantation. In humans too, VPA, CBZ and OXC might contribute to infertility and abortion risks