Upregulated lncRNA-Malat1 During Different Time Points of Epileptogenesis
Abstract number :
1.012
Submission category :
1. Basic Mechanisms / 1A. Epileptogenesis of acquired epilepsies
Year :
2018
Submission ID :
501625
Source :
www.aesnet.org
Presentation date :
12/1/2018 6:00:00 PM
Published date :
Nov 5, 2018, 18:00 PM
Authors :
Lei Chen, West China Hospital, Sichuan University; Xiangmiao Qiu, West China Hospital, Sichuan University; Lin Zhang, West China Hospital, Sichuan University; Wanlin Lai, West China Hospital, Sichuan University; Anjiao Peng, West China Hospital, Sichuan U
Rationale: Long non-coding RNAs (lncRNAs) are a novel class of non-coding transcripts, which had been identified in various regions of central nervous system. Now, there have been no report about the role of lncRNAs in epileptogenesis. This research aims to find out whether lncRNAs are associated with epileptogenesis. Methods: Epileptogenesis was induced in 8 wistar male rats (250g-270g) by injecting pilocarpine intraperitoneally. Two rats were administered 0.9% NS for control. In two time points of epileptogenesis, 1 week and 2 weeks after status epilepsia, four rats were executed respectively and lncRNA microarray was used to identify differentially expressed lncRNA. Bioinformatics analysis were later done to reveal the possible mechanism of lncRNA in epileptogenesis. Results: Several differentially expressed lncRNA were identified, among which lncRNA-Malat1 expression was found to be markedly upregulated in different time point of epileptogenesis. Further bioinformatics analysis suggested that lncRNA-Malat1 may play its regulation mechanism on target gene-erk by phosphorylating SR protein. Conclusions: In our previous study, lncRNA-Malat1 facilitate neural outgrowth. Based on this results, we hypothesized that lncRNA-Malat1 might activate mossy fiber sprouting and epileptogenesis by regulating gene-erk. Funding: This work has been supported by Sichuan Province Science and Technology Project (2017HH0103).