Abstracts

It is known that neurogenesis still continues in the dentate gyrus even after reaching adulthood. Several reports have described the neurogenesis from surgical specimens of temporal lobe epilepsy, not only in an animal epilepsy model. However, the function of neurogenesis remains to elucidate. We herein examined the neurogenesis in the human hippocampus from temporal lobe epilepsy patients by immunohistochemical staining, and compared the neurogenesis between the specimens from hippocampal sclerosis and non-hippocampal sclerosis., Six surgical specimens were examined by immunohistochemical staining using NeuroD, PSA-NCAM, GFAP, Ki-67. Three cases thus demonstrated hippocampal sclerosis in both magnet resonance imaging and pathological evaluations, and another 3 showed non-hippocampal sclerosis. The cell counting of positive cells of each type of staining was applied in the ammon horn and the dentate gyrus. We also observed the nature of the cell and axon with positive staining., PSA-NCAM positive cells were mostly detected in the hilus, and also in the granule cell and CA1 pyramidal cell layers in both the hippocampal sclerosis and non-hippocampal sclerosis groups. The PSA-NCAM positive immature neurons were co-stained with NeuN, but not with either NeuroD or Ki-67. The PSA-NCAM positive immature neurons were frequently detected in the non-hippocampal sclerosis group., The PSA-NCAM and NeuN co-stained immature neurons were located in the hilus of human temporal lobe epilepsy. However, the ability of neurogenesis was found to be higher in the non-hippocampal sclerosis group than in the hippocampal sclerosis group.
Figure 1[figure1], (Supported by Juntendo University.)