2DE-DIGE Proteomic Analysis in Mesial Temporal Lobe Epilepsy
Abstract number :
4.232
Submission category :
Human Genetics
Year :
2006
Submission ID :
7121
Source :
www.aesnet.org
Presentation date :
12/1/2006 12:00:00 AM
Published date :
Nov 30, 2006, 06:00 AM
Authors :
1Marcelo J. Murai, 2Ricardo Horiuchi, 2Daniel Martins, 1Cl[aacute]udia Maurer-Morelli, 2José Camillo Novello, 3Fernando Cendes, and 1Iscia Lopes-Cendes
Mesial temporal lobe epilepsy (MTLE) is the most common and severe type of partial epilepsy, representing [sim]50% of all adult epilepsy patients and frequently associated with pharmaco-resistance. The relationship between MTLE and hippocampal sclerosis (HS) is well established. However, the precise pathogenesis of HS and its relationship with MTLE is not completely clarified. Two-dimensional electrophoresis (2DE) is a powerful fractionation method for complex protein mixtures. In difference gel electrophoresis (DIGE) based proteomics, the experimental and control samples are labeled with different fluorophores and are run in the same gel, thereby minimizing gel preparation variation. DIGE is one of the few techniques that is capable to perform quantitative proteomics, generating statistical data to differences in protein abundances., We analyzed the proteome of three hippocampus removed from patients with refratory MTLE who underwent epilepsy surgery., 2DE-DIGE identified 2 up-regulated and 10 down-regulated proteins as determined by Student[apos]s T-test ([italic]p[/italic][le]0.01). The observed molecular weight of the spots ranged from 28 to 93 kDa., The identity of these spots will be elucidated by mass spectrometry in order to gain additional information in a global scale about the mechanism of epileptogenesis in MTLE., (Supported by Fapesp, CAPES and CNPq.)
Genetics